Next, the barcode information is used to computationally assign the sequence reads back to the individual libraries. Libraries can then be pooled and sequenced simultaneously. To distinguish individual libraries throughout this process, sample-specific sequences, called sample indexes or sample barcodes, are added to each fragment during library preparation. As a result, libraries are commonly pooled together and sequenced simultaneously via a process known as multiplexing. The capacity of next generation sequencing (NGS) platforms has increased at an astonishing rate.
Drug target identification via CRISPR screening.